Staff Research Associate 3 - Pediatric BMT

• Department of Pediatrics is seeking a highly skilled and motivated Senior Research Associate III (SRA) to support our research project on T cell development, thymic epithelial biology, and thymic regeneration. The SRA will serve as a senior technical specialist leading in vivo rodent research projects on thymic and T cell functions. The ideal candidate will possess extensive knowledge in T cell and thymus biology, experience independently managing large, complex mouse colonies, planning and performing long-term, complex surgical procedures, and executing downstream tissue, cellular and molecular analyses, with exceptional attention to detail. The SRA is also required to independently plan and carry out original research projects in the laboratory, using a combination of techniques including cell culture, FACS, Western blot, immunohistochemistry, confocal microscopy, and molecular biology. As an SRA, the candidate is expected to operate with a high level of independence, troubleshoot technical difficulties, and maintain safety and regulatory compliances. The ideal candidate needs to demonstrate excellent interpersonal skills, and assist in training students and junior lab personnel. DUTIES & ESSENTIAL JOB FUNCTIONS % of time Essential Function (Yes/No ) Key Responsibilities (To be completed by Supervisor) 50 Yes Mouse colony management and experimental operations include:
• Independently manage large, complex immunodeficient mouse colonies including NSG-athymic mice and other NSG strains.
• Perform routine animal husbandry tasks including breeding, weaning, genotyping and tagging; monitor colony health; coordinate with vivarium staff regarding logistics.
• Plan and execute long-term, complex dosing procedures including HSC engraftment and immunization; plan and perform blood collection; conduct survival surgeries including renal capsule transplantations, and post-op animal care.
• Conduct terminal tissue collection under aseptic conditions, including thymus, spleen, bone marrow and grafted tissues.
• Work with BSL2+ materials in animals.
• Oversee animal record keeping, including colony records, surgery logs and controlled substance use logs. 25 Yes Wet lab operations include:
• Confocal microscopy: prepare tissue sections, perform IHC, IF and ICC staining, and execute confocal imaging.
• FACS: using multi-color panels, including surface and intracellular staining.
• Molecular biology: perform DNA/RNA extractions, PCR/qPCR, western blot, molecular cloning, and NGS library preps.
• Cell culture: maintain primary and cell line cultures, perform transfections, transductions, and AAV and lentivirus preps.
• Work independently to optimize and develop new protocols to address novel scientific questions. Be expected to use scientific innovation in developing new and novel approaches to the research. 15 Yes Data analysis and reporting include:
• Process, analyze and organize experimental data using specialized software.
• Maintain meticulous lab notebooks and digital records.
• Assisting the PI in drafting/updating IACUC/IBC protocols, writing manuscripts and presentations, preparing NIH/CIRM documentations. 10 Yes Lab managerial duties include:
• Ensure lab compliance with safety and regulatory guidelines.
• Assist with lab organization including electronic and paper record keeping.
• Oversee the maintenance, inspection and repair of lab equipment.
• Provide training and guidance to junior lab members, students and trainees. 0 0 0 100% (To update total %, enter the amount of time in whole numbers (without the % symbol - e.g., 15, 20) then highlight the total sum (e.g., 1%) at the bottom of the column and press F9. The total sum should add up to 100%.) Required

BA/BS with a degree in a related science, including two years of recent/directly related work experience, utilizing the techniques/methods required by the position and demonstrating technical expertise in these areas; and/or equivalent experience/training Background in immunology, with specific knowledge in hematopoiesis and T cell development Demonstrated proficiency in mouse husbandry, breeding schemes, and multiple administration routes Proven ability to perform rodent survival surgery and tissue collection under aseptic conditions Experience with a wide range of wet lab skills, including cell culture, FACS, microscopy, and molecular biology techniques Proficiency with specialized scientific software such as FlowJo, ImageJ/LAS X, and GraphPad Prism Excellent organizational skills, strong record-keeping, and ability to work independently as a technical specialist

Specialized scientific background in thymus development and TEC/thymocyte crosstalks Demonstrated surgical techniques including bone marrow transplantation, renal capsule transplantation, and adoptive transfer models Extensive experience with advanced multi-color FACS including spectral flow cytometry Experience working with BSL2+ materials Prior experience managing vendor relations, ordering, and compliance documentation (IACUC, EH&S, BSL-2)

Full time · Day shift